Figure 1

TCR repertoire diversity in gluten exposed CD patients. (a) No difference in repertoire diversity is observed between gluten specific CD4+ IFNg+sorted TCRB repertoires and pre- (day0) or post- challenge (day6) CD patient PBMC repertoires. Gut repertoires show interesting increase in diversity (decrease in clonality) during gluten exposure (Wilcoxon paired Signed Rank test, two-tailed, p = 0.06; paired t-test, two-tailed, p = 0.0077. The p-value from the t-test is shown here since the Wilcoxon signed rank test has limitations when applied to small sample size even though it is the main test employed in this study). Repertoire samples were downsampled to 27077 reads 100 times, each time estimating diversity. For each sample, median of the 100 diversity estimates is used for plotting and statistical comparison. Horizontal bars indicate median. (b) The diversity increase in active CD gut repertoires ranged from 1.9 to 7.9% (median: 6%) of the treated gut repertoire diversity in patients.