Figure 5 | Scientific Reports

Figure 5

From: Deep sequencing of blood and gut T-cell receptor β-chains reveals gluten-induced immune signatures in celiac disease

Figure 5

Public differentially enriched CD patient PBMC clonotypes. (a) DA clonotypes are enriched in post-challenge CD patient samples (color intensity indicates prevalence level), their prevalence in healthy control pre- and post-challenge samples is also shown for comparison. The donut plot on the right shows the TCRBV genes used by these DA clonotypes in CD patient post-challenge samples, from the least to the most used. Clonotypes were hierarchically clustered using a modified Levenshtein distance that took physicochemical properties into account; the cluster indicated with the green bar has the highest average fold change increase during gluten exposure. (b) MEME motif search of the top cluster: significantly over-represented motif shown in sequence logo and regular expression, ASS[LF]R[SW][TD][DT][TE][QA][YF] (E-value = 2.4E-10); Top cluster contains previously known HLA-DQ2 restricted, alpha gliadin epitope specific CD4+ clonotypes with the ASSxRxTDTQY motif (highlighted with light green).

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