Figure 7

Functional reconstitution of detergent purified SbMATE. Representative recordings for the purified SbMATE recombinant protein expressed in P. pastoris reconstituted into liposomes and incorporated into lipid bilayers in an asymmetric ionic cis:trans gradient (200: 20 mM NaCl, pH 7.5). (A) Representative current recording following addition of SbMATE containing proteolipomes to the cis side of the artificial lipid bilayer. O and arrows indicate open states. C and dashed lines indicate the zero current closed state. Time scale is denoted on the bottom margin. The zero current level (closed state) in all cases is indicated by the horizontal dotted lines. (B) All point histograms illustrating the open and close state distributions for the full length recordings illustrated in (A). The zero current level (closed state) and various open states are above each peak.