Figure 2
From: Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis
Deoxycorticosterone acetate (DOCA)-salt treatment induced tubulointerstitial fibrosis. (A) Representative images of Masson trichrome-stained kidney sections of mice under control conditions and DOCA-salt-treated conditions for 2 days (DOCA-2), 4 days (DOCA-4), and 8 days (DOCA-8). DOCA-treated mice showed gradually aggravated interstitial fibrosis (blue). The area of tubulointerstitial fibrosis, as well as the entire cortical area, in 10 high-power fields was measured with ImageJ software. (B) The degree of tubulointerstitial fibrosis was evaluated as a ratio relative to the entire cortical area, n = 8. (C–E) RT-PCR analysis of the fibrosis-related gene (transforming growth factor β1, Tgf-β1; collagen type I alpha1, Col1a1; fibronectin 1, Fn1) expression in the kidneys of the controls and mice treated with DOCA-salt for 2, 4, and 8 days, n = 5–6. **P < 0.001 compared to the controls, 5 s rRNA is used as an internal control, error bars, mean ± SD.
