Figure 7
From: High-Temperature Requirement A1 (Htra1) - A Novel Regulator of Canonical Wnt Signaling
The activity of HTRA1 may be dependent on its tertiary structure. COS7 cells were transfected with GFP, HTRA1-GFP, or HTRA1-HA, along with mCherry or FLAG-dvl (1 μg). Forty-eight hours after transfection, cells were fixed, permeabilized, and stained with an anti-FLAG or anti-HA antibody. Alexa-546 and Alexa-488 were used as fluorescent antibodies, GFP and HTRA1-GFP were detected through the GFP tag, and mCherrry was detected through the cherry tag. Cells were visualized using confocal microscopy.
