Figure 1
From: CRTC1 mediates preferential transcription at neuronal activity-regulated CRE/TATA promoters
Neuronal activity induces CREB-dependent gene expression in primary cortical neurons. Quantitative real-time RT-PCR analysis of mRNA levels of CREB target genes in cultured cortical neurons (10 DIV). Levels of c-fos, Dusp1, Nr4a1, Nr4a2 and Ptgs2 mRNAs were differentially increased after KCl, FSK or FSK/KCl treatment in a time-dependent manner. On the contrary, Gapdh mRNA levels were not significantly different between vehicle- (Veh) and FSK/KCl-treated neurons at any time point, while Psen1 and Psen2 mRNA levels were significantly changed only after 8 hours of stimulation. Data represent fold change ± s.e.m relative to vehicle (Veh)-treated neurons from three independent experiments (n = 3). Gene expression levels were normalized to the geometric mean of Ppia, Tbp and Gapdh. N.s: non-significant. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the indicated experimental group as determined by two-way ANOVA followed by Bonferroni test.
