Figure 2 | Scientific Reports

Figure 2

From: Two novel mutations identified in ADCC families impair crystallin protein distribution and induce apoptosis in human lens epithelial cells

Figure 2

Molecular verification of transfected cells. (A) CRYBB2 relative expression level in untransfected and transfected cells. WT, wild-type cells; WT-E, cells expressing EGFP; Crybb2-WT, cells expressing normal CRYBB2; Crybb2-mut, cells expressing mutant CRYBB2. (B) CRYAA relative expression level in normal cells and transfected cells. WT, wild-type cells; WT-E, cells expressing EGFP; Cryaa-WT, cells expressing normal CRYAA; Cryaa-mut, cells expressing mutant CRYAA. GAPDH was used as a housekeeping gene. (C) Western blot analysis of βB2-crystallin in normal cells and transfected cells. 1, wild-type cells; 2, cells expressing EGFP; 3, cells expressing normal βB2-crystallin; 4, cells expressing mutant βB2-crystallin; GAPDH was used as the internal control. The original western blot images are shown in Supplementary Fig. S2 and S3. (C) Western blot analysis of αA-crystallin in normal cells and transfected cells. 1, wild-type cells; 2, cells expressing EGFP; 3, cells expressing normal αA-crystallin; 4, cells expressing mutant αA-crystallin, GAPDH was used as the internal control. The original western blot images are shown in Supplementary Fig. S4 and S5.

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