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Figure 1

From: Piperonylic acid stimulates keratinocyte growth and survival by activating epidermal growth factor receptor (EGFR)

Figure 1

EGFR was activated by piperonylic acid. (a) The modified EGFP-SH2 biosensor protein was well expressed with the expected size in transfected A549 cells (expected molecular weight of a duplicate of SH2 domains is 20 kDa). (b) The modified biosensor system detected EGFR activation shown in bright green fluorescent spots. (c) The modified biosensor system recognized EGFR activation upon piperonylic acid treatment. (d) Tyrosine phosphorylation of EGFR was increased by piperonylic acid treatment (10 minutes). Scale bar, 50 μm. Blue fluorescence represents nucleus.

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