Figure 1 | Scientific Reports

Figure 1

From: Spred2 Deficiency Exacerbates D-Galactosamine/Lipopolysaccharide -induced Acute Liver Injury in Mice via Increased Production of TNFα

Figure 1

D-GalN/LPS-induced liver injury. WT and Spred2−/− mice were i.p. injected with D-GalN (400 mg/kg)/LPS (20 µg/kg). (a) Serum ALT levels were measured after D-GalN/LPS challenge (3–10 mice, each time point). *P < 0.05, **P < 0.01 vs. WT mice. (b) Left, representative photographs of liver HE sections at indicated time after D-GalN/LPS challenge are shown. The scale bar indicates 50 µm. Right, injured area with haemorrhage at 6 h after D-GalN/LPS challenge was semi-quantitated (6 mice, each). *P < 0.05 vs. WT-livers. (c) Hepatic leukocytes at 3 h after D-GalN/LPS challenge were isolated from WT (6 mice) and Spred2−/−-livers (8 mice), and stained with PE-Cy7 anti-mouse CD45 (leukocytes), FITC anti-mouse Ly6G and PerCP/Cy5.5 anti-mouse CD11b (neutrophils), or PE anti-mouse F4/80 and PerCP/Cy5.5 anti-mouse CD11b (macrophages), and analysed by flow cytometry. *P < 0.05 vs. WT mice.

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