Figure 2 | Scientific Reports

Figure 2

From: Spred2 Deficiency Exacerbates D-Galactosamine/Lipopolysaccharide -induced Acute Liver Injury in Mice via Increased Production of TNFα

Figure 2

Hepatocyte apoptosis in D-GalN/LPS-induced liver injury. WT and Spred2−/− mice were i.p. injected with D-GalN (400 mg/kg)/LPS (20 µg/kg), and the mice were sacrificed at 5 h after injection. (a) Left, representative photographs of liver sections with TUNEL staining are shown. The scale bar indicates 50 µm. Right, the numbers of TUNEL-positive hepatocytes/HPF were counted in WT-livers (5 mice) and Spred2−/−-livers (6 mice). *P < 0.05 vs. WT-liver. (b) Activities of caspase-3, caspase-8 and caspase-9 in WT-livers and Spred2−/−-livers (10 mice, each group). *P < 0.05, ***P < 0.001 vs. WT-livers. (c) mRNA expressions of FasL in WT-livers and Spred2−/−-livers (10 mice, each group) were analysed by RT-qPCR. The expression levels of each mRNA were normalized to that of GAPDH. *P < 0.05 vs. WT-livers. (d) Liver extracts were immunoblotted using the indicated primary antibodies. Upper, representative immunoblot data from two independent analyses of lysates from different mice. Lower, band densities were digitised and semi-quantitated. Full-length blots/gels are presented in Supplementary Figure 2. *P < 0.05 vs. WT-livers.

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