Figure 3 | Scientific Reports

Figure 3

From: Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells

Figure 3

Imaging flow cytometry analysis. (a–c) Settings for analysis. Histograms with the corresponding bright field and fluorescence images of single cells. Dead cells are stained red employing 7-AAD. (a) Histogram shows cells out of focus (a) and in focus (b). Only cells in focus were used for further analysis. (b) In a next step, single cells were selected (c). 7-AAD negative debris (d) and cell agglomerates (e) were excluded from further analysis. (c) The Histogram shows viable ((f) 7-AAD negative) and non viable ((g) 7-AAD positive) cells. Scale bars equal 10 µm. Cell viability of (d) mEPCs and (e) hECFCs on protein-coated scaffolds using trypan blue or imaging flow cytometry with 7-AAD (n = 3).

Back to article page