Table 1 HILIC-DMS-MS/MS parameters for analysis of BMAA and proteogenic amino acids.
Analyte | Retention Time (min) | Compensation Voltage (V) | Precursor ion m/z | Product ion m/z | Collision Energy (V) |
|---|---|---|---|---|---|
BMAA | 15.7 | −23.5 | 119 | 102 | 13 |
44 | 25 | ||||
13C15N2-BMAA | 15.7 | −23.5 | 122 | 105 | 13 |
47 | 25 | ||||
D3-BMAA | 15.7 | −23.1 | 122 | 104 | 13 |
46 | 25 | ||||
Phenylalanine | 6.3 | −14.4 | 166 | 120 | 17 |
Leucine | 6.4 | −15.5 | 132 | 43 | 33 |
Isoleucine | 6.6 | −16.6 | 132 | 69 | 21 |
Methionine | 7.2 | −18.3 | 150 | 104 | 15 |
Valine | 7.5 | −18.6 | 118 | 72 | 17 |
Tyrosine | 7.8 | −13.0 | 182 | 165 | 14 |
Proline | 7.9 | −22.3 | 116 | 70 | 23 |
Alanine | 9.1 | −21.4 | 90 | 44 | 18 |
Glutamic acid | 9.9 | −21.0 | 148 | 84 | 20 |
Glycine | 10.0 | −21.9 | 76 | 30 | 20 |
Cysteine | 10.1 | −22.7 | 122 | 76 | 14 |
Threonine | 10.1 | −22.9 | 120 | 74 | 15 |
Aspartic acid | 10.1 | −20.9 | 134 | 74 | 19 |
Serine | 11.1 | −23.5 | 106 | 60 | 16 |
Histidine | 17.4 | −15.8 | 156 | 110 | 20 |
Arginine | 17.7 | −18.0 | 175 | 70 | 30 |
Lysine | 18.1 | −17.6 | 147 | 84 | 23 |
