Figure 2 | Scientific Reports

Figure 2

From: C-reactive protein and pentraxin-3 binding of factor H-like protein 1 differs from complement factor H: implications for retinal inflammation

Figure 2

Contribution of the FHL-1 C-terminal tail to CRP binding. Recombinant CCP67 proteins (i.e. 402Y and 402 H variants with and without the 4-amino acid C-terminal tail of FHL-1) were used at a range of concentrations to determine their binding to immobilized monomeric CRP (mCRP) or pentameric CRP (pCRP) in plate assays. (A) Binding of the 402Y isoform of CCP67 constructs with/without tail to immobilized mCRP. Differences in binding at the two highest concentrations are statistically significant (Student T test, * = p < 0.05). (B) Binding of the 402Y isoform of CCP67 constructs with/without a tail to immobilized pCRP. (C) Binding of the 402 H isoform of CCP67 constructs with/without a tail to immobilized mCRP. (D) Binding of the 402 H isoform of CCP6-7 constructs with/without a tail to immobilized pCRP. Data (mean ± SEM) are combined from two identical experiments performed in quadruplicate (n = 8).

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