Figure 3 | Scientific Reports

Figure 3

From: C-reactive protein and pentraxin-3 binding of factor H-like protein 1 differs from complement factor H: implications for retinal inflammation

Figure 3

The C-terminal tail of FHL-1 contributes to PTX3 binding. In plate assays, PTX3 was used in the fluid phase to determine its binding to immobilized FH/FHL-1 proteins: (A) FH; (B) FHL-1: (C) CCP67 402Y ( ± tail); and (D), CCP67 402 H ( ± tail). (E) The maximum binding values obtained from the experiments in (A–D) were re-plotted to highlight the significant effects between 402Y and 402 H forms of CCP67 ± tail; here statistical analysis (Student’s T test) compared pairs of data as shown (**p < 0.01, ****p < 0.0001). All data (mean ± SEM) are combined from two identical experiments performed in quadruplicate (n = 8) for FH and FHL-1, and three identical experiments performed in quadruplicate (n = 12) for CCP67 constructs. The binding curves in B are statistically different when analysed by two-way ANOVA, p = 0.0025, and the binding at the top three individual concentrations are statistically different where *p < 0.001 (Student’s T test).

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