Figure 3
From: Beta-catenin cleavage enhances transcriptional activation
Inhibitors of chymotrypsin-like activity of the proteasome diminish β-catenin transcriptional activity. (A) HT29 cells transfected with TCF/LEF reporter were treated with inhibitors as indicated. Luciferase activity is shown in cells incubated with protease inhibitors. Asterisks indicate statistically significant p values compared to control. p < 0.001. (B) HT29 cells were treated as in A and nuclear chromatin-bound fractions probed for pβ-Cat552. Fibrillarin served as a loading control. Full size membrane scans for WBs can be seen in Suppl. Fig. SS3.
