Table 2 Primers and probes designed and used in droplet digital PCR.
Gene | Direction | Sequence | Length (bp) | Tm (°C) | GC% |
|---|---|---|---|---|---|
16S | Probe (Fam) | TATTGGGCGTAAAGGGTGCGTAGG | 100 | 68 | 54.0 |
Forward | GCAGCCGCGGTAATACATA | 62 | 52.6 | ||
Reverse | GAGCATTGCACTTAGACCATAAAC | 62 | 41.7 | ||
Bn Actin-2 | Probe (Hex) | TGCTGGATTCTGGTGATGGTGTGT | 94 | 72 | 50.0 |
Forward | CAGTGGTCGTACTACTGGTATTG | 68 | 47.8 | ||
Reverse | GATGGCGTGTGAAAGAGAGA | 60 | 50.0 |
