Figure 5
From: Connexin 43 Hemichannel as a Novel Mediator of Sterile and Infectious Inflammatory Diseases
P5 significantly attenuated the LPS-induced ATP release and trypan blue dye uptake in macrophage cultures. (a) P5 peptide attenuated the LPS-induced ATP release. *P < 0.05 versus “+LPS”. (b,c) P5 peptide significantly suppressed the LPS-induced trypan blue dye uptake. RAW 264.7 cells were stimulated with LPS in the absence or presence of P5 peptide for 16 h, and stained with trypan blue dye. Phase contrast images were randomly captured from multiple fields (Panel c), and the percentage of trypan blue-stained cells were calculated (Panel b). *P < 0.05 versus “−LPS”; # P < 0.05 versus “+LPS”.
