Figure 5
From: Pneumolysin induced mitochondrial dysfunction leads to release of mitochondrial DNA
Pneumolysin induces extracellular release of mtDNA. (A) Relative quantification of mitochondrial DNA released from A549 cells in the supernatant after stimulation with the indicated amounts of PLY after three hours. Data are expressed as amount of mitochondrial DNA normalized to unstimulated cells (ctrl). Bars represent mean ± SD from n = 5 independent experiments, *P < 0.05, **P < 0.01; Kruskal-Wallis test with Dunn’s post-hoc test). (B) Quantification of mitochondrial DNA released from A549 control cells compared to ρlow A549 cells in the supernatant. Cells were stimulated with 1.0 µg/ml PLY for three hours. Bars represent mean ± SD from n = 3 independent experiments, *P < 0.05, one-tailed Mann-Whitney test). (C) Quantification of mitochondrial DNA within microvesicles released from A549 stimulated with 0.05 and 0.10 µg/ml PLY for four hours. Bars represent mean ± SD from n = 5 independent experiments, *P < 0.05, Kruskal-Wallis test with Dunn’s post-hoc test. (D) Quantification of mitochondrial DNA released from human lung tissue stimulated with 5.0 µg/ml PLY, 5 U/ml DNase or 5.0 µg/ml PLY + 5 U/ml DNase for eight hours. Bars represent mean ± SD from n ≥ 8 independent experiments, *P < 0.05, **P < 0.01; Kruskal-Wallis test with Dunn’s post-hoc test). (D) Quantification of IL-1β released from human lung tissue stimulated with 5.0 µg/ml PLY, 5 U/ml DNase or 5.0 µg/ml PLY + 5 U/ml DNase for eight hours. Bars represent mean ± SD from n ≥ 8 independent experiments, ***P < 0.001; Kruskal-Wallis test with Dunn’s post-hoc test).
