Table 3 Comparison of engineered and parent phage-derived peptides.

From: Polypharmacy through Phage Display: Selection of Glucagon and GLP-1 Receptor Co-agonists from a Phage-Displayed Peptide Library

Peptide

Sequence

EC50 hGCGR (nM)

EC50 hGLP1 (nM)

11

HSQGTFTSDY       SKYLDSRRAHDFVQWLLNT

0.018 ± 0.005

0.018 ± 0.001

36

HsQGTFTSDK(γEγEC16) SKYLDSRRAHDFVQWLLNT

0.023 ± 0.004

0.050 ± 0.006

16

HSQGTFLSDY     SKLLDSRAAQDFVQWLMQT

0.131 ± 0.060

11.22 ± 10.56

37

HsQGTFLSDK(γEγEC16) SKLLDSRAAQDFVQWLMQT

0.037 ± 0.004

23.48 ± 7.599

  1. 1All peptides C-terminal carboxyamide; s = D-Ser; γE = γ-glutamic acid, C16 = palmitic acid; bold, changes from the native GCG sequence.
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