Figure 2
M. tuberculosis induces differentially expressed transcripts during in vitro infection of whole blood. (a) Volcano plot showing transcripts differentially expressed in response to infection of whole blood with M. tuberculosis over time (0–96 h) in the discovery data set (using blood from 4 individual donors). Blue dots (n = 2,858) represent significantly differentially expressed (SDE) transcripts between matched infected and uninfected samples, and with |maximum log2FC| < 1; red dots (n = 1,026) represent SDE transcripts with |maximum log2FC| > 1. (b) Validation of changes in mRNA expression; correlation of |maximum log2FC| between SDE transcripts in the discovery and validation study (top 1000 transcripts shown). (c) Heat map showing timing of maximum mRNA expression of up- (red) and down- (green) regulated genes for the top 1000 transcripts with |maximum log2FC| > 1 in M. tuberculosis infected whole blood as compared to uninfected whole blood. Black represents no difference in expression. The relative degree of transcript abundance is indicated by the colour intensity derived from the fitted mean expression levels over time (see methods).
