Table 1 Bacterial strains and plasmids used in this study.

From: Characterisation of the antibacterial properties of the recombinant phage endolysins AP50-31 and LysB4 as potent bactericidal agents against Bacillus anthracis

Strain or plasmid

Relevant characteristics

Reference or source

Strains

E. coli

BL21

Host for recombinant endolysin expression

Novagen (Madison, WI, USA)

Non- anthracis Bacillus

Bacillus cereus ATCC 4342

Isolate from milk

ATCC

Bacillus circulans ATCC 21783

Isolate from soil

ATCC

Bacillus laevolacticus ATCC 23492

Isolate from Ranunculus sceleratus

ATCC

Bacillus licheniformis KCOM 1491

Isolate from human subgingival dental plaque

KCOM

Bacillus megaterium ATCC 10778

Isolate from soil

ATCC

Bacillus pumilus KCTC 3713

Isolate from air-conditioner filter of subway station

KCTC

Bacillus subtilis RIK 1285

Bacillus expression host strain

Takara (Kusatsu, Japan)

Bacillus thuringiensis BGSC 4AA1

Wild type isolate

BGSC

Bacillus thuringiensis BGSC 4AJ1

Wild type isolate

BGSC

Bacillus thuringiensis BGSC 4BA1

Wild type isolate

BGSC

Bacillus thuringiensis BGSC 4CC1

Wild type isolate

BGSC

B. anthracis

ΔSterne

pXO1− pXO2−

Hanyang University (Seoul, Republic of Korea)

Sterne

pXO1+ pXO2−

KCDC

ATCC 14578

pXO1+ pXO2+

KCDC

HYU01

pXO1+ pXO2+

Hanyang University49

Plasmids

pBAD::SAL-1

Backbone vector used for the construction of pBAD-AP50-31 and pBAD-B4

46

pBAD-AP50-31

Endolysin AP50-31 expression plasmid

This study

pBAD-B4

Endolysin LysB4 expression plasmid

This study

  1. ATCC, American Type Culture Collection (Manassas, VA, USA); KCOM, Korean Collection for Oral Microbiology (Gwangju, Republic of Korea); KCTC, Korean Collection for Type Cultures (Jeongeup, Republic of Korea); BGSC, Bacillus Genetic Stock Center (Columbus, OH, USA); KCDC, Korea Centers for Disease Control and Prevention (Osong, Republic of Korea).
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