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Figure 1

From: Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia

Figure 1

Changes in cell growth and viability between normoxia and hypoxia. (A) The cell growth of keratinocytes derived from cholesteatomas and (B) the external auditory canal. (C) Cell viability of each keratinocyte type cultured in normoxia and 24-h hypoxia was measured using the WST-1 test. (D) Concurrent detection of changes in the mitochondrial membrane potential was determined using JC-1 dye. The data shown are pooled from two patients. The results represent the combined data of two separate experiments and are expressed as the mean ± standard error of the mean (SEM), with n = 4 for each bar. *indicates p < 0.05; **indicates p < 0.005; CK = cholesteatoma keratinocyte; EACK = external auditory canal keratinocyte.

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