Figure 2
Comparison of the intracellular ATP levels and lactate contents of cholesteatoma keratinocytes and external auditory canal keratinocytes cultured in normoxia and hypoxia for 24 h. (A) Both types of keratinocytes after 24-h culture under different oxygen tensions, the cells (2 × 105) were lysed for the determination of cellular ATP levels using an ATP Bioluminescence Assay Kit. (B) Culture medium (50 μl) of each sample after 24-h culture under different oxygen tensions were harvested to measure the lactate content using the Lactate Colorimetric Assay Kit. The data shown are pooled from two patients. The results represent the combined data of two separate experiments and are expressed as the mean ± standard error of the mean (SEM), with n = 4 for each bar. *indicates p < 0.05.
