Figure 3 | Scientific Reports

Figure 3

From: Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia

Figure 3

Mitochondrial density differences between the two types of keratinocytes cultured in normoxic and hypoxic conditions. (A) Representative images of MitoTracker Green FM staining for the detection of changes in mitochondrial mass in the external auditory canal keratinocytes cultured in normoxia and (B) 24 h of hypoxia. (C) MitoTracker Green FM staining of cholesteatoma keratinocytes cultured in normoxia and (D) 24-h of hypoxia. Nuclei were labeled using Hoechst 33342 (blue). (E) Quantification and comparison of mitochondrial densities. The results are expressed as the mean ± standard error of the mean (SEM) with n = 4 for each bar. **indicates p < 0.005.

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