Figure 4 | Scientific Reports

Figure 4

From: Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia

Figure 4

Exposure of EAC keratinocytes to 5% O2 for 24 h induced a significant increase in autophagy. (A) Representative images of LC3 punctum formation for the detection of autophagy in external auditory canal keratinocytes and cholesteatoma keratinocytes cultured in 20% and 5% O2 for 24 h, respectively. (B) Quantification and comparison of LC3 punctum formation. The results are expressed as the mean ± standard error of the mean (SEM) with n = 4 for each bar. **indicates p < 0.005.

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