Figure 4

Theoretically vs. experimentally determined number of lipid molecules/liposome (A) and number of particles/mL (B) during the extrusion process. The number of HSPC molecules that could be loaded per liposome was estimated from the surface area of HSPC:Chol liposomes (considering the average size of the vesicles determined by DLS or NTA) divided by the area occupied by the lipid polar head. The number of HSPC molecules was also taken from the number of particles – determined by NTA – divided by the number of HSPC molecules in 0.125 mM suspension (7.5 × 10¹⁶ molecules).