Figure 6

Mitochondrial shape 10 min after CCCP treatment evaluated by FIB-SEM tomography. Fifty-three randomly selected mitochondria (of a total of 226) from MEFs are shown (a). The shape of the mitochondria was categorised into seven groups, with adjacent images showing solid and translucent views of the surface rendering to observe invaginations. The proportion belonging to each group is shown in chart (b), and most mitochondria showed a vase shape (123/226). Structural characteristics of the orifice diameter (c) and the thinnest thickness of the mitochondrial matrix (d) in vase-shaped mitochondria are shown in histograms. Scale bar, 1 μm.