Figure 8 | Scientific Reports

Figure 8

From: In vitro and in vivo antitumor potential of carvacrol nanoemulsion against human lung adenocarcinoma A549 cells via mitochondrial mediated apoptosis

Figure 8

Apoptotic potential of CANE against PC-9 cells. (a,b) MTT and LDH assay, respectively, of CANE (25–150 μg/ml)-treated PC-9 cells after 24 h of incubation. Docetaxel (20 μg/ml) was used as a positive control while the surfactant-polysorbate 80 mix devoid of carvacrol was also used as a control. (c) Morphological changes in PC-9 cells after treatment with CANE (25–125 μg/ml). Images were captured at 20X magnification [scale bar = 0.1 mm]. (d) Effect of CANE on expression of JNK, Bax, Bcl2, Cyt C, caspase-9, and 3 in PC-9 cells. (e) Cellular levels of protein markers JNK, p-JNK, Bax, Bcl2, Cyt C, caspase-3, caspase-9, and β- actin in PC-9 cells incubated with Mito-TEMPO (10 μM). Densitometry analysis of the respective proteins was evaluated by Image J software, and results were normalized with β-actin with respect to controls. Each value in the bar graph represents the mean ± SD of three independent experiments. Values with different superscripts differ significantly from each other (p < 0.05).

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