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Figure 1

From: Flavin Reductase Contributes to Pneumococcal Virulence by Protecting from Oxidative Stress and Mediating Adhesion and Elicits Protection Against Pneumococcal Challenge

Figure 1

Expression of recombinant Flavin reductase (rFlaR). (a) rFlaR was purified using Ni-NTA beads under denaturing conditions and resolved by SDS-PAGE using sample buffer with or without β-mercaptoethanol (β-ME). (b) rFlaR was separated using SDS-PAGE. The protein was transferred to a nitrocellulose membrane and probed with rabbit antiserum raised against rFlaR (lane 1) or preimmune rabbit serum (lane 2). (c) Expression of FlaR on the surface of R6 was detected by flow cytometry using mouse anti-rFlaR antibodies. (d) Cytoplasmic (cyto) and cell wall (CW) fractions prepared from WU2 were subjected to electrophoresis and immunoblotted with rabbit anti-rFlaR antiserum. Of note, this gel was cropped from Supplementary Fig. S2.

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