Figure 5 | Scientific Reports

Figure 5

From: Flavin Reductase Contributes to Pneumococcal Virulence by Protecting from Oxidative Stress and Mediating Adhesion and Elicits Protection Against Pneumococcal Challenge

Figure 5

Fe2+ involvement in FlaR enzymatic activity. (a) Different divalent ion chelators (EDTA (10 nM), NTA (10 nM) and citrate (10 nM)) introduced into rFlaR reaction mixture significantly inhibited rFlaR redox enzymatic activity. Results are presented as Δ A340 nm, that was calculated as described in Fig. 3 The results presented are the mean of three different experiments (one way ANOVA with Dunnett’s post hoc multiple comparisons test, n = 3 in each group, ****p = 0.0001). (b) The effect of the H2O2 on rFlaR NADP-reductase activity was eliminated by H2O2 scavenger, salicylate (Spearman correlation, r = −0.0513, p = 0.5 not significant).

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