Figure 2 | Scientific Reports

Figure 2

From: Lack of Fgf18 causes abnormal clustering of motor nerve terminals at the neuromuscular junction with reduced acetylcholine receptor clusters

Figure 2

Fgf18 is expressed in both the spinal cord and the diaphragm. (A) Representative immunostaining for FGF18 (green) and choline acetyltransferase (ChAT, red) expressed in SMNs of the adult spinal cord. A region indicated by a white square is enlarged in panels B-D. Note that signals for FGF18 and ChAT are co-localized in SMNs (arrowheads). *Central canal. Bar = 100 μm. (B–D) High magnification of the squared region in (A). Expressions of indicated proteins are shown in (B) and (C). FGF18 and ChAT are shown in green and red in (D). Bar = 10 μm. (E) Localizations of FGF18 and AChR in a cross section of the tibialis anterior muscle in adult and of proximal muscles of lower extremities at E18.5. Co-localization of AChR (red) and FGF18 (green) is indicated in the right-most merged images. Arrows point to AChR clusters in each panel. Bar = 20 μm.

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