Table 2 Mutant oriD inverted complementary repeat (ICR) sequences.

From: oriD structure controls RepD initiation during rolling-circle replication

  1. Mutant oriD oligoduplexes were synthesized and then ligated into the wild type (wt) pCerOriD plasmid to yield three oriD variants mut2, mut3 and mut2/3. The following symbols are used; “:” identical to wt; “|” delimits the three ICRs, bold indicates hairpin stems (see Fig. S3C); shaded regions are the restriction enzyme sites (HindIII and AvaI) used to resect the wild type pCerOriD plasmid so the synthesized oligoduplexes could be inserted and religated.
Back to article page