Figure 5
From: Mice deficient in the Shmt2 gene have mitochondrial respiration defects and are embryonic lethal
Characterization of MEF lines generated from 12.5-dpc embryos with no mutation (Shmt2+/+; Gcat+/+) and with homozygous mutations (Shmt2 m/m; Gcat m/m). (a) Genotyping of the mutation. (b) Western blot analysis of SHMT2 protein and GCAT protein. (c) Biochemical analysis of relative enzymatic activities of mitochondrial respiratory complexes. (d) Western blot analysis of the subunits of mitochondrial respiratory complexes encoded by mtDNA (ND1, COX1) and nuclear DNA (NDUFA9, COX4, SDHA). (e) Doubling times in culture. Experiments were performed in triplicate. Data are means ± s.e.m. *P < 0.05, **P < 0.01, Student’s t-test.
