Figure 3
Assessment of in vivo transmission blockade following immunization with PbPfs25DR3 ookinetes by DFA. To assess transmission-blocking activity following immunization, 5 mice per regime (experimental and control) were infected/challenged with P. berghei PbPfs25DR3 and three days later, mosquitoes were exposed to individual mice to perform DFA. DFAs were performed in three individual tranches to account for varying experimental timings within multiple regimes (A) regimes 1,2,7,8; (B) regimes 3,4,9,10; (C) regimes 5,6,11,12). Transmission blockade was assessed as mean reduction in oocyst intensity/ prevalence with respect to cohorts of non-immunized challenged mice within each DFA. Regimes 5 and 6 contain four mice due to death before challenge. Individual data points represent the number of oocysts found in individual mosquitoes 12 days post feed. Horizontal bars indicate mean intensity, whilst error bars indicate S.E.M. For each regime, mean reductions in intensity and prevalence for each group are reported in Table 1.
