Figure 8

Validation of RNA-seq data by qRT-PCR analysis. A total of 22 genes involved in different biological processes that showed differential response to CySNO in RNA-seq analysis were selected for confirmation through qRT-PCR. These genes include those involved in (a) growth and development, (b) abiotic stress, (c) protein degradation, (d) signalling, (e) secondary metabolite production, and (f) plant defence. The correlation coefficient (R) of 0.951 indicates that qRT-PCR results are significantly correlated with RNA-seq analysis. Each data point is the mean of three replicates and error bars represents standard error (±SE).