Table 1 Primer sequences used in QRT-PCR analysis.
Symbol | Description | Primer Sequences | Product (bp) |
|---|---|---|---|
GCG | Glucagon | F: 5′-GGCACATTCACCAGCGACTACA-3′ | 174 |
R: 5′-GCCCTCCAAGTAAGAACTCACATC-3′ | |||
GLP1R | glucagon-like peptide 1 receptor | F: 5′-TCAGAGACGGTGCAGAAATG-3′ | 183 |
R: 5′-CAGCTGACATTCACGAAGGA-3′ | |||
HPRT | Hypoxanthine guanine phosphoribosyl transferase | F: 5′-CGTCGTGATTAGCGATAGTG-3′ | 178 |
R: 5′-ACAGAGGGCCACAATGTGAT-3′ | |||
INS1 | insulin I | F: 5′-CCTTAGTGACCAGCTATAATCAGAG-3′ | 248 |
R: 5′-CACTTGTGGGTCCTCCACTT-3′ | |||
INS2 | insulin II | F: 5′-GAAGTGGAGGACCCACAAGTG-3′ | 112 |
R: 5′-GATCTACAATGCCACGCTTC-3′ |
