Table 3 Real values for each variables in the 2nd stage of factorial design.

From: Microbial synthesis of a useful optically active (+)-isomer of lactone with bicyclo[4.3.0]nonane structure

Components

Level

La

0

Hb

Content of components [g/L]

(NH4)H2PO4

5

7.5

12

KH2PO4

3.5

7

10.5

Glucose

50

60

70

NaCl

0.025

0.05

0.05

Minerals

CuSO4 × 5H2O

0.01

0.01

0.02

ZnSO4 × 7H2O

0.12

0.12

0.24

MnSO4 × 4H2O

0.02

0.02

0.04

MgSO4 × 7H2O

0.8

1

1.2

  1. aLow-content; bHigh-content.
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