Table 1 Results of the HRM speciation assay tested on 113 Gram-negative bacterial isolates compared with a reference standard of 16S rRNA PCR and sequencing, by individual target.
HRM | Reference test - 16S PCR and sequencing | total | |||||||
|---|---|---|---|---|---|---|---|---|---|
Ab | Ec | S | Kp | Ko | Pa | 16S | Negative | ||
A. baumannii | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
E. coli | 0 | 37 | 0 | 0 | 0 | 0 | 0 | 0 | 37 |
Salmonella sp. | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
K. pneumoniae | 0 | 0 | 0 | 25 | 0 | 0 | 0 | 0 | 25 |
K. oxytoca | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
P. aeuruginosa | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 3 |
16S | 0 | 0 | 0 | 0 | 0 | 0 | 99 | 0 | 99 |
Negative | 0 | 0 | 0 | 2 | 0 | 0 | 14 | 608 | 624 |
Total | 1 | 37 | 1 | 27 | 1 | 3 | 113 | 609 | 791 |
