Figure 1 | Scientific Reports

Figure 1

From: Gene and MicroRNA Perturbations of Cellular Response to Pemetrexed Implicate Biological Networks and Enable Imputation of Response in Lung Adenocarcinoma

Figure 1

Schematic diagram of analyses and experiments performed. The primary analysis workflow starts with the differential expression analysis in LCLs following drug treatment (orange box), progressing to identification of inversely correlated miRNA-mRNA pairs, eQTL analysis in human lungs for the differentially expressed mRNAs, functional annotation enrichment (DAVID) analysis, and protein-protein interaction analysis (green circles). The subsequent validation performed includes mRNA replication using an independent array, plus the qPCR in treated and untreated A549 cells, apoptosis and cytotoxicity assay in A549 cells upon drug treatment, and TCGA profile analysis of mRNAs (gray circles).

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