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Figure 1

From: A Single Dose of Modified Vaccinia Ankara expressing Ebola Virus Like Particles Protects Nonhuman Primates from Lethal Ebola Virus Challenge

Figure 1

MVA-EBOV vaccine construction and expression. (a) Schematic of MVA-EBOV vaccine expressing EBOV-like particles. I8R and G1L, conserved vaccinia sequences flanking the insertion site for gp; A50R and B1R, conserved sequences flanking the insertion site for vp40. PmH5, modified early late H5 vaccinia promoter. Numbers indicate positions in the MVA genome which is abbreviated and not to scale. (b) Western blot showing expression of EBOV GP (monoclonal Ab c6D8) and VP40 (polyclonal Ab) in 293 T cell lysates (LYS) and supernatants (SUP). Molecular weight markers are indicated to the left of blots. (c) Thin section electron micrograph showing the expression of EBOV-like particles. Arrows in (c) show examples of immunogold staining for GP. (d) Magnified thin section electron micrograph showing the immunogold staining of GP (black dots) without arrows.

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