Table 2 Steady state kinetics of D-2-CPA dehalogenation by HadD AJ1 and its mutants. aDifference in transition state free energies between WT and mutant enzymes. ΔΔGES* = −RTln[(kcat/Km)WT/(kcat/Km)mutant]34.

From: Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures

Enzymes

WT

V51F

N203A

D205E

Km (mM)

0.94

2.90

12.00

4.13

kcat (min−1)

981.00

757.00

35.40

330.00

(kcat/Km)WT/(kcat/Km)mutant

—

4.02

355.28

13.12

ΔΔGES*(kJ/mol)a

—

−3.50

−14.80

−6.49

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