Figure 3
Temperature-dependent modulation of fly behavior by Gr28bD. (A) Incapacitation by temperature: flies overexpressing GR28b genes had their locomotor activity recorded for 90 seconds at 24–40 °C in two-degree steps. The plot shows the cumulative proportion of flies not incapacitated at each temperature. Most flies with pan-neuronal or motor neuron overexpression of GR28bD were temporarily incapacitated at 34 or 36 °C, respectively. Gr28bB overexpression had an effect at the very high end of the tested temperature range. (B) Control and Gr28bD-overexpressing flies were exposed to 32–40 °C in one-degree steps. Most flies overexpressing Gr28bD pan-neuronally were incapacitated at 33–35 °C. The heterozygous genetic control flies did not show incapacitation over the tested temperatures. Incapacitation is faster and recovery is slower at higher exposure temperatures. (C) Example position traces of a non-incapacitated fly (top panel) and of an incapacitated fly that recovered (bottom trace). The red area represents the exposure to high temperature and the arrows mark the times of incapacitation and recovery. (D) Time to incapacitation at different temperatures: flies overexpressing Gr28bD and flies heterozygous for the GAL4 driver and UAS-Gr28bD construct were tested. Most flies with Gr28bD pan-neuronal overexpression were incapacitated within 10 seconds at 36, 38, and 40 °C, but it took much longer at 34 °C. Few flies from the control genotypes were incapacitated at the temperatures tested. (E) Time to recovery from incapacitation: flies recovered faster when subjected to lower temperature. In A, B, and D, statistical difference between control and experimental genotypes was determined with a Kaplan-Meier test, p < 0.000001 = ****. In each experiment, 60–80 flies of each genotype were tested.
