Figure 3
From: Malaria infected red blood cells release small regulatory RNAs through extracellular vesicles
Profiling of human miRNAs and tRNAs isolated from P. falciparum infected EVs. (a) Top 16 most highly expressed unique miRNAs in EVs. Left axis and bars: percent of total miRNA reads that mapped to each of the top 16 most abundant miRNAs. Error bars are standard deviation (n = 3). Right axis and line: cumulative percent of total miRNA reads. (b) Significantly (Benjamini Hochberg corrected P value < 0.05) enriched KEGG pathways for target genes. The number of miRNA involved in targeting at least one gene in a given pathway are shown in parentheses. (c) Distribution of human pre-mature miRNAs (n = 3). (d) Distribution of known tRNA sequences based on the total read counts. tRNA sequences are categorized by number of read counts corresponding to each individual tRNA (n = 3). (e) Based on the small RNA-seq results, only tRNA fragments were identified in EVs. Shown are examples of tRNA structures for each species depicting the boundaries of the identified tRNA fragment. The read density at each position is shown as a heatmap.
