Figure 7
From: Malaria infected red blood cells release small regulatory RNAs through extracellular vesicles
Transfer of plasmodial RNAs to endothelial cells via EVs. (a) Bone marrow endothelial cells (BMECs) were incubated with 100 μg of PKH67 fluorescently labelled EVs for 0 or 6 h. BMECs were stained for actin (phalloidin, red) and nuclei (DAPI, blue). Scale bar, 10 μm. (b) Endothelial cells were incubated with 100 μg of EVs for 12 hours. After isolation of their RNA, the transfer of plasmodial RNA was measured by qPCR. The average and S.D. of 3 experiments is shown. (mean ± s.e.m.; n = 3 experiments), ***P < 0.0001 versus control (Student’s t-test). (c) Functional classification of the identified Plasmodium RNAs in EVs by gene ontology (GO) analysis conducted using Cytoscape with the Cluego plugin. This highlights the regulatory function, which plasmodial RNAs may exert in the recipient cells. (c) GO molecular function (d) GO cellular localization (e) GO biological function.
