Table 1 Five steps to obtain Pochonia chlamydosporia strain 170 chromosome sequences.

	Step 1	Step 2	Step 3	Step 4	Step 5	PC123^a
Chromosomes	—	—	—	—	7	—
scaffold Number	184	114	100	70	49	894
scaffold Size (bp)	43,858,609	43,842,403	43,931,568	44,216,750	44,215,803	41,979,339
scaffold N50 (bp)	4,091,865	4,627,976	4,615,841	4,614,946	5,359,152	225,463
contig Number	631	548	316	114	114	8,512
contig Size (bp)	43,494,633	43,443,055	43,741,514	44,192,108	44,192,108	40,759,443
contig N50 (bp)	211,032	232,386	477,820	1,984,628	1,984,628	14,640
GC content (%)	49.5	49.5	49.5	49.5	49.5	49.9
Repeat sequences (%)	—	5.5	—	7.9	7.9	1.8
Gene Number	—	14,867	—	14,204	14,204	12,122^b
Secreted Proteins	—	1,782	—	1,750	1,750	1,530
Small secreted proteins	—	743	—	728	728	654
SCS proteins^c	—	153	—	157	157	116

Note: 1. Step 1: Obtained by Allpaths-LG assembly.
2. Step 2: Obtained by comparative analysis of Allpaths-LG assembly and SSPACE-LongRead assembly.
3. Step 3: Obtained by performing Jelly implemented in PBSuite based on Canu assembly.
4. Step 4: Obtained by performing Jelly based on the SMRT sequencing data that had been corrected by LoRDEC, and performing Pilon improvement and Tablet for manual correction.
5. Step 5: Obtained by comparative analysis of step4 assembled results and Canu assembly.
6. ^aThe genome sequence of P. chlamydosporia 123.
7. ^bAmong the 12,122 genes, 138 genes may be error predicted due to more stop codons found for one gene sequences, and these 138 genes were not used to predict secreted proteins.
8. ^cSCS proteins, small cysteine-rich secreted proteins.

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