Figure 1
From: A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption
SPD inhibits Rhl regulated pyocyanin production by P. aeruginosa PA01. (A) Biofilm co-aggregation observed in the no treatment PA01 culture was not observed in SPD treated 48-72 hours cultures, n = 6. (B) Bar graph showing mean levels of pyocyanin in control and SPD treated samples. Data are shown mean ± SD, n = 6, *p < 0.05. (C) The total ion signal chromatograms of pyocyanin and internal standard norharmane produced by P. aeruginosa PA01 in normal condition (con) and in the presence of compound (SPD). The retention times for pyocyanin and norharmane with the solvent system used in this study were 8.1 min and 9.25 min, respectively. The total ion signal chromatograms of pyocyanin and norharmane (internal standard) produced by P. aeruginosa PA01 in normal condition (con) and in the presence of compound (SPD). The retention times for pyocyanin and norharmane with the solvent system used in this study were 8.1 min and 9.25 min, respectively. (D) P aeruginosa PA01 was either left untreated or treated for 24 h with SPD. mRNA was isolated and real-time PCR was performed to assess rhlR gene expression in PA01.
