Figure 1

Identification of Aggrecan enhancers. (A) ENCODE analysis of the mouse Acan locus. Peaks show strong indication of enhancers using H3K4me1 and H3K27ac along with conservation. The selected regions for analysis are highlighted, +28, −10(A1), −30, −62 and −80. (B) Schematic of the HSP68-LacZ-GW reporter vector that was used to create transgenic mice for analysis. (C) Representative whole mount images of transgenic embryos at E15.5 for each enhancer region tested showing a variation of transgene activity