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Figure 1

From: Maternal whole blood cell miRNA-340 is elevated in gestational diabetes and inversely regulated by glucose and insulin

Figure 1

Criteria for participant selection and work flow. Whole blood was collected from eight normal glucose tolerant (NGT) and eight gestational diabetes (GDM) pregnant women for genome-wide transcriptome analysis (screening group). The samples were matched based on age and body mass index (BMI) and were derived from the pregnancy cohort. After whole blood RNA isolation, sequencing (Illumina), library preparation and biostatistical analysis (miRlastic) was conducted. Thirty NGT and thirty GDM women were selected for q-PCR experiments (validation group). Quantification of miRNA-340 only was conducted in lymphocytes collected from pregnant NGT and GDM women (N = 38) (lymphocyte donors). From this group either lymphocyte cell culture or protein quantification was conducted. Additionally, cord blood samples were collected from eight children from NGT pregnancies (mNGT) and eight from GDM pregnancies (mGDM). Finally, cell culture and miRNA-340 quantification was conducted in lymphocytes of non-pregnant controls.

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