Figure 6

Tissue localization of MUL-MIR482A and measurement of pri-mul-miR482a and mul-miR482a-5p. (A) GUS staining in MUL-MIR482 promoter::reporter transgenic plants. (B and C) Measurement of pri-mul-miR482a (B) and mature mul-miR482a-5p (C) in various tissues of mulberry, respectively. Pri-mul-miR482a and mul-miR482a-5p abundance were detected by RT-qPCR, and relative abundance was evaluated using comparative Ct method using actin (Accession No. DQ785808) and U6 as the reference, respectively. Values are given as the mean ± SD of three experiments in each group.