Figure 5
From: Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells
Morphology, size, and structure characterizations of MVs produced by dynamic 3D hMSC-culture. (a) TEM images of 3D-MVs. Most collected MVs displayed vesicular structures appearing rounded and bi-lipid layered, although differing in contrast and surface pattern. The size bars indicate 2,000 nm (left) and 500 nm (right). (b) Size distribution of the collected MVs obtained by measuring the sizes of individual vesicles on multiple TEM images (approximately 1,400 vesicles from 11 images). A peak in the MV diameter range was found at approximately 250–300 nm with over 80% of the collected MVs populated between 150 and 450 nm. (c) Particle size standards for flow cytometry obtained using the Nano Fluorescent Size Standard Kit. (d) A major population of the collected MVs was superimposed on the size calibration plot mainly in the range between 220 and 450 nm. (e and f) Flow cytometry results of the collected MVs before (e) and after (f) Triton-X 100 treatment, which indicated that the collected MVs were true lipid-membranous vesicles.
